Visualizing My Microbiome

     

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1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

1000x. My own saliva stained with methylene blue. Personal buccal epithelial cells are visible as large pale blue cells with a dark staining nucleus (my genome lives here). Numerous bacteria are visible either attached to the cells or in other parts of the stained sample.

Paul Ehrlich’ made countless contributions to science,  in fields as diverse as histology, haematology, immunology, oncology, microbiology and pharmacology. In the course of his investigations Ehrlich came across methylene blue, which he regarded as particularly suitable dye  for staining bacteria. Here I have drawn upon Ehrlich’s early studies on staining bacteria and developed a simple off-the shelf/ DIYBio-staining procedure for bacteria and  human cells. It is based on methylene blue which is readily available as a “fish medicine”. The brand I used here is King British Methylene Blue. It works very well as it comes in the bottle, and without the need for any messy preparation. Here I have stained saliva from my own mouth and the stain has revealed my own cells, their nuclei, and the normal bacterial flora of my mouth

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