I’m optimizing conditions that incease light output in the bioluminescent bacteria, Photorhabdus luminescens and Photorhabdus asymbiotica. Interesting that conditions that promote light production in P. luminescens diminish production of the orange pigment.
Before art there is always science. These are experiments to optimize light output in the bioluminescent bacteria Photorahbdus asymbiotica and Photorhabdus luminescens by changing the incubation temperature, and by adding various carbon sources to the media. The effect of temperature is dramatic. Incubation at 37 C, also human body temperature, results in the best light production. Also the colonies are brightest without any additional carbon source and thus the carbon sources used here at least repress light output. Unlike the bioluminescent marine bacteria such as Photobacterium phosphoreum, the light produced by the Photorhabdus species here is stable at higher temperatures and does not require the addition of high concentrations of salt and offers alternative artistic applications.